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KMID : 0381120230450070921
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Genes and Genomics
2023 Volume.45 No. 7 p.921 ~ p.934
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Genome-wide identification of the valine-glutamine motif containing gene family and the role of VQ25-1 in pollen germination in Brassica oleracea
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Marziehsadat Razavizadegan
Ziwei Liu
Yuanhui Yu
Min Yang
Li Guo
Xuejie Han
Xiangjie Ma
Ziya Huang
Qiguo Gao
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Abstract
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Background : The plant-specific valine-glutamine (VQ) motif containing proteins tightly regulate plant growth, development, and stress responses. However, the genome-wide identification and functional analysis of Brassica oleracea (B. oleracea) VQ genes have not been reported.
Objective : To identify the VQ gene family in B. oleracea and analyze the function of Bo25-1 in pollen germination.
Methods : The Hidden Markov Model (HMM) of VQ family was used to query the BoVQ genes in the B. oleracea genome. The BoVQ genes preferentially expressed in anthers were screened by qRT-PCR. Subcellular localization of VQ25-1 was observed in Nicotiana benthamiana (N. benthamiana) leaves. To analysis the role of BoVQ25-1 in pollen germination, the expression of BoVQ25-1 was suppressed using antisense-oligonucleotides (AS-ODN).
Results : A total of 64 BoVQ genes were identified in the B. oleracea genome. BoVQ25-1 was found to be preferentially expressed in the B. oleracea anthers. BoVQ25-1 was cloned from the anthers of the B. oleracea cultivar ‘Fast Cycle’. BoVQ25-1 is localized to the nucleus. The pollen germination rate significantly decreased after AS-ODN treatment.
Conclusion : Sixty-four BoVQ genes were identified in the B. oleracea genome, of which BoVQ25-1 plays an important role in pollen germination.
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KEYWORD
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Genome-wide Identification, B. oleracea, VQ gene family, VQ25-1, Pollen germination
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